precast polyacrylamide gels (Bio-Rad)

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Bio-Rad precast polyacrylamide gels
Precast Polyacrylamide Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/precast+polyacrylamide+gels/4%E2%80%9315%25+Mini-PROTEAN+TGX+Stain-Free+Protein+Gels/pmc13657230-479-14-17
Average 96 stars, based on 971 article reviews

precast polyacrylamide gels - by Bioz Stars, 2026-10

96/100 stars

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Electrophoresis:

Article Title: Sex and ovarian hormone cycles alter effects of stimulant drugs on mouse dopaminergic signaling
Article Snippet: SDS-PAGE: 6 micrograms of protein in RIPA buffer were mixed with 4x Laemmli Sample Buffer (BIO-RAD 1610747: 3 parts lysate 1 part buffer) supplemented with 10% 2-mercaptoethanol and boiled at 95 degrees Celsius for 5 minutes. .. Samples were loaded onto 4–20% precast polyacrylamide gels (BIO-RAD 5671094) and proteins separated by electrophoresis. .. Western Blotting: Proteins were transferred onto 0.45 micrometer pore PVDF membranes (Thermo Scientific B88585) at 100 volts for 30 minutes at 4 degrees Celsius in SDS-free transfer buffer containing 20% methanol.

Staining:

Article Title: Puf4 is methylated and exhibits a temperature-dependent interactome in Cryptococcus neoformans .
Article Snippet: .. Elution volumes were separated on 4%–15% Mini-PROTEAN TGX stain-free precast polyacrylamide gels (Bio-Rad) at 150 V. Gels were stained with Coomassie blue, and bands corresponding to Puf4-FLAG were excised. ..

Article Title: Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity
Article Snippet: Laemmli sample buffer (Bio-Rad) containing dithiothreitol (DTT) was added to each sample and samples were mixed and heated (e.g. 10 minutes at 70°C) before loading. .. A total of 10ug of protein per lane was loaded onto 15% TGX Stain-Free precast polyacrylamide gels (Bio-Rad, Cat. No. 4568086) and proteins were separated by SDS-PAGE. .. Following electrophoresis, proteins were transferred onto suitable membranes using the Trans-Blot transfer system (Bio-Rad, Cat. No. 1704156), following the manufacturer’s recommendations.

Article Title: Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairment
Article Snippet: For analysis of cytosolic proteins, the cells were lysed in 1% Triton X-100 (Thermo Fisher Scientific) in PBS supplemented with cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitors (Roche). .. An equal volume or amount of the samples was loaded to a 4 to 15% Mini-Protean TGX stain-free precast polyacrylamide gels (Bio-Rad) and Precision Plus ProteinTM All Blue Standards (Bio-Rad) was used as a ladder. .. The total protein content of the supernatant was determined with the Pierce BCA protein assay kit (BCA) protein assay (Thermo Fisher Scientific).

Nucleic Acid Electrophoresis:

Article Title: IMPDH inhibition enhances cytarabine efficacy in SAMHD1-expressing leukaemia cells via guanine nucleotide depletion.
Article Snippet: Samples of total 10–20 lg protein were prepared in NuPAGE LDS Sample Buffer 49 (no. NP0007; Thermo Fisher Scientific) and NuPAGE Sample Reducing Agent 10X (no. NP0004; Thermo Fisher Scientific) and denatured at 95 °C for 7 min. .. Sample lysates and protein standards (Precision Plus Protein Dual Color Standards, no. 1610374; Bio-Rad, Hercules, CA, USA) were loaded into Criterion TGX 4–20% precast polyacrylamide gels (Bio-Rad) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and further transfer to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). ..

Membrane:

Article Title: Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stress
Article Snippet: .. Proteins were separated using 4-20% precast polyacrylamide gels (Bio-Rad, Cat #5671094) and transferred to a PVDF membrane using the TurboBlot transfer system (Bio-Rad, Cat #1704150). .. After transfer, blots were incubated in 5% non-fat milk in TBS (Tris-buffered Saline, Bio-Rad, Cat #1706435), supplemented with 0.1% Tween-20 (Thermo Fisher Scientific, Cat #28320) for 1.5 hours at RT.

Protein Electrophoresis:

Article Title: Heterologous expression of fungal glycoside hydrolase family 115 α-glucuronidases.
Article Snippet: Deconstruction of plant xylan is challenging due to the structural complexity arising from side groups on the polymer.. One of the side groups, glucuronic acid, can be further modified with a methyl group at the 4-hydroxy position, as well as modification by the addition of sugar, acetate, and/or ferulate.. This heterogeneity necessitates multiple enzymes capable of recognizing and hydrolyzing each side group type for complete hydrolysis of xylan to xylose.

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